| Pos. | Nr. | Fahrer | Team | Motorrad | Zeit | auf F. | Abstand | Rdn. |
|---|---|---|---|---|---|---|---|---|
| 1 | 27 | C. Stoner | Ducati | Ducati | 1:58.002 | 21 | ||
| 2 | 21 | J. Hopkins | Suzuki | Suzuki | 1:58.653 | 0.651 | 22 | |
| 3 | 14 | R. de Puniet | Kawasaki | Kawasaki | 1:58.939 | 0.937 | 0.286 | 22 |
| 4 | 5 | C. Edwards | Yamaha | Yamaha | 1:59.231 | 1.229 | 0.292 | 21 |
| 5 | 65 | L. Capirossi | Ducati | Ducati | 1:59.347 | 1.345 | 0.116 | 22 |
| 6 | 71 | C. Vermeulen | Suzuki | Suzuki | 1:59.359 | 1.357 | 0.012 | 21 |
| 7 | 50 | S. Guintoli | Tech3 | Yama | 1:59.460 | 1.458 | 0.101 | 22 |
| 8 | 26 | D. Pedrosa | Honda | Honda | 1:59.465 | 1.463 | 0.005 | 23 |
| 9 | 1 | N. Hayden | Honda | Honda | 1:59.516 | 1.514 | 0.051 | 23 |
| 10 | 6 | M. Tamada | Tech3 | Yamaha | 1:59.590 | 1.588 | 0.074 | 20 |
| 11 | 4 | A. Barros | Pramac | Ducati | 1:59.712 | 1.710 | 0.122 | 19 |
| 12 | 46 | V. Rossi | Yamaha | Yamaha | 1:59.849 | 1.847 | 0.137 | 21 |
| 13 | 7 | C. Checa | LCR | Honda | 1:59.892 | 1.890 | 0.043 | 22 |
| 14 | 24 | T. Elias | Gresini | Honda | 2:00.089 | 2.087 | 0.197 | 17 |
| 15 | 56 | S. Nakano | Scot-Honda | Honda | 2:00.353 | 2.351 | 0.264 | 21 |
| 16 | 13 | A. West | Kawasaki | Kawasaki | 2:00.472 | 2.470 | 0.119 | 24 |
| 17 | 80 | K. Roberts | KR-Ducati | KR | 2:00.595 | 2.593 | 0.123 | 20 |
| 18 | 22 | I. Silva | Pramac | Ducati | 2:01.362 | 3.360 | 0.767 | 18 |
| Pos. = Position, Rnd. = Runden | ||||||||
Tschechien / Brünn
3. Freies Training
