| Pos. | Nr. | Fahrer | Team | Motorrad | Zeit | auf F. | Abstand | Rdn. |
|---|---|---|---|---|---|---|---|---|
| 1 | 99 | J. Lorenzo | Yamaha | Yamaha | 1:49.870 | 27 | ||
| 2 | 46 | V. Rossi | Yamaha | Yamaha | 1:50.065 | 0.195 | 27 | |
| 3 | 27 | C. Stoner | Ducati | Ducati | 1:50.144 | 0.274 | 0.079 | 23 |
| 4 | 3 | D. Pedrosa | Honda | Honda | 1:50.808 | 0.938 | 0.664 | 24 |
| 5 | 5 | C. Edwards | Tech3 | Yamaha | 1:50.891 | 1.021 | 0.083 | 25 |
| 6 | 15 | A. de Angelis | Gresini | Honda | 1:50.898 | 1.028 | 0.007 | 24 |
| 7 | 65 | L. Capirossi | Suzuki | Suzuki | 1:51.007 | 1.137 | 0.109 | 24 |
| 8 | 14 | R. de Puniet | LCR | Honda | 1:51.030 | 1.160 | 0.023 | 26 |
| 9 | 4 | A. Dovizioso | Honda | Honda | 1:51.206 | 1.336 | 0.176 | 26 |
| 10 | 33 | M. Melandri | Hayate | Kawasaki | 1:51.359 | 1.489 | 0.153 | 22 |
| 11 | 52 | J. Toseland | Tech3 | Yamaha | 1:51.401 | 1.531 | 0.042 | 24 |
| 12 | 24 | T. Elias | Gresini | Honda | 1:51.823 | 1.953 | 0.422 | 22 |
| 13 | 7 | C. Vermeulen | Suzuki | Suzuki | 1:51.994 | 2.124 | 0.171 | 25 |
| 14 | 88 | N. Canepa | Pramac | Ducati | 1:52.319 | 2.449 | 0.325 | 23 |
| 15 | 69 | N. Hayden | Ducati | Ducati | 1:52.403 | 2.533 | 0.084 | 25 |
| 16 | 72 | Y. Takahashi | Scot-Honda | Honda | 1:52.607 | 2.737 | 0.204 | 24 |
| 17 | 36 | M. Kallio | Pramac | Ducati | 1:52.619 | 2.749 | 0.012 | 25 |
| Pos. = Position, Rnd. = Runden | ||||||||
Italien / Mugello
1. Freies Training
