| Pos. | Nr. | Fahrer | Team | Motorrad | Zeit | auf F. | Abstand | Rdn. |
|---|---|---|---|---|---|---|---|---|
| 1 | 99 | J. Lorenzo | Yamaha | Yamaha | 1:56.331 | 23 | ||
| 2 | 3 | D. Pedrosa | Honda | Honda | 1:56.462 | 0.131 | 24 | |
| 3 | 46 | V. Rossi | Yamaha | Yamaha | 1:56.787 | 0.456 | 0.325 | 24 |
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| 4 | 5 | C. Edwards | Tech3 | Yamaha | 1:57.058 | 0.727 | 0.271 | 23 |
| 5 | 65 | L. Capirossi | Suzuki | Suzuki | 1:57.384 | 1.053 | 0.326 | 20 |
| 6 | 24 | T. Elias | Gresini | Honda | 1:57.495 | 1.164 | 0.111 | 23 |
| 7 | 4 | A. Dovizioso | Honda | Honda | 1:58.008 | 1.677 | 0.513 | 25 |
| 8 | 36 | M. Kallio | Ducati | Ducati | 1:58.060 | 1.729 | 0.052 | 19 |
| 9 | 15 | A. de Angelis | Gresini | Honda | 1:58.111 | 1.780 | 0.051 | 22 |
| 10 | 69 | N. Hayden | Ducati | Ducati | 1:58.252 | 1.921 | 0.141 | 22 |
| 11 | 33 | M. Melandri | Hayate | Kawasaki | 1:58.368 | 2.037 | 0.116 | 23 |
| 12 | 52 | J. Toseland | Tech3 | Yamaha | 1:58.413 | 2.082 | 0.045 | 24 |
| 13 | 88 | N. Canepa | Pramac | Ducati | 1:58.518 | 2.187 | 0.105 | 23 |
| 14 | 7 | C. Vermeulen | Suzuki | Suzuki | 1:58.551 | 2.220 | 0.033 | 24 |
| 15 | 14 | R. de Puniet | LCR | Honda | 1:58.567 | 2.236 | 0.016 | 20 |
| 16 | 84 | M. Fabrizio | Pramac | Ducati | 1:59.457 | 3.126 | 0.890 | 16 |
| 17 | 41 | G. Talmacsi | Scot-Honda | Honda | 1:59.645 | 3.314 | 0.188 | 24 |
| Pos. = Position, Rnd. = Runden | ||||||||
Tschechien / Brünn
2. Freies Training
