| Pos. | Nr. | Fahrer | Team | Motorrad | Zeit | auf F. | Abstand | Rdn. |
|---|---|---|---|---|---|---|---|---|
| 1 | 99 | J. Lorenzo | Yamaha | Yamaha | 1:56.299 | 11 | ||
| 2 | 46 | V. Rossi | Yamaha | Yamaha | 1:56.714 | 0.415 | 10 | |
| 3 | 3 | D. Pedrosa | Honda | Honda | 1:57.208 | 0.909 | 0.494 | 9 |
| 4 | 5 | C. Edwards | Tech3 | Yamaha | 1:57.340 | 1.041 | 0.132 | 10 |
| 5 | 24 | T. Elias | Gresini | Honda | 1:57.417 | 1.118 | 0.077 | 10 |
| 6 | 15 | A. de Angelis | Gresini | Honda | 1:57.697 | 1.398 | 0.280 | 10 |
| 7 | 65 | L. Capirossi | Suzuki | Suzuki | 1:57.711 | 1.412 | 0.014 | 10 |
| 8 | 14 | R. de Puniet | LCR | Honda | 1:57.946 | 1.647 | 0.235 | 10 |
| 9 | 69 | N. Hayden | Ducati | Ducati | 1:57.967 | 1.668 | 0.021 | 10 |
| 10 | 4 | A. Dovizioso | Honda | Honda | 1:58.019 | 1.720 | 0.052 | 10 |
| 11 | 36 | M. Kallio | Ducati | Ducati | 1:58.158 | 1.859 | 0.139 | 10 |
| 12 | 52 | J. Toseland | Tech3 | Yamaha | 1:58.425 | 2.126 | 0.267 | 10 |
| 13 | 33 | M. Melandri | Hayate | Kawasaki | 1:58.591 | 2.292 | 0.166 | 10 |
| 14 | 7 | C. Vermeulen | Suzuki | Suzuki | 1:58.888 | 2.589 | 0.297 | 10 |
| 15 | 88 | N. Canepa | Pramac | Ducati | 1:58.938 | 2.639 | 0.050 | 10 |
| 16 | 84 | M. Fabrizio | Pramac | Ducati | 1:59.780 | 3.481 | 0.842 | 9 |
| 17 | 41 | G. Talmacsi | Scot-Honda | Honda | 1:59.854 | 3.555 | 0.074 | 9 |
| Pos. = Position, Rnd. = Runden | ||||||||
Tschechien / Brünn
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